Cat. No.
MABL-1796
Application
ELISA, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
1D3
From
Recombinant Antibody
Specificity
The antibody is specific to metal-substituted protoporphyrin. CDRH2 plays an essential role in recognition. The antibody has the highest affinity for PP[Fe]-PEG and a five-times lower affinity for PP[Pd]- PEG, PP[Zn]-PEG, and PP[Ga]-PEG.
Alternative Names
Panhematin; Hematin
UniProt
Immunogen
This antibody was raised against a hemin-human immunoglobulin in BALB/c mice.
Application Notes
The affinity of this antibody for hemin has been characterized by ELISA (Uda et al., 1993). The affinity constant of the antibody and individual CDR segments has been examined with fluorescence measurement of protoporphyrin-PEG binding (Hifumi et al., 1999). The mouse IgM version of this antibody (Ab00982-21.0) was used as a primary antibody for immunocytochemistry (IHC) in myelinating cultures after treatment with hemin/FeCl3 (Baldacchino et al., 2022; PMID: 35569511).
Antibody First Published
Uda et al. Production and immunological characterization for anti hemin monoclonal antibody Chem Lett. 1993;22(11):1923-1926. doi: 10.1246/cl.1993.1923.
Note on publication
The original publication describes the generation of a monoclonal antibody for hemin and the characterization of its protoporphyrin binding.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
