Cat. No.
MABL-1797
Application
WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Cow, Horse, Mammals, Pig, Sheep
Clone No.
13F7
From
Recombinant Antibody
Specificity
13F7 recognizes a hemoglobin monomer (14 Da) in blood samples of various mammals (pig, sheep, horse, cow).
Alternative Names
haemoglobin monomer; Hb; Hbg; PHb; mAb13F7
UniProt
Immunogen
The original mouse IgG1 version of this antibody was raised by immunizing BALB/c mice with a mammalian blood immunogen.
Application Notes
This antibody is recommended for the identification of hemoglobin and, more generally, blood in various samples and products, such as commercial meat products. It was used in the development of a successful ELISA-based assay for the quantification of hemoglobin (PHb) in meat products. It had a very wide working range from 0.5 ppm to 1000 pp, high sensitivity and precision (Jiang et al., 2018; pmid: 29412908). Testing by Western blot demonstrated that 13F7 is able to detect a hemoglobin monomer (mass: 14 Da) in blood samples of different mammals (Jiang et al., 2018; pmid: 29412908). Consequently, one of the potential areas where this antibody will be particularly useful is the food industry and food control against adulteration with meat products.
Antibody First Published
Jiang et al. Monoclonal antibody-based ELISA for the quantification of porcine hemoglobin in meat products Food Chem. 2018 Jun 1;250:170-179. doi: 10.1016/j.foodchem.2018.01.032. Epub 2018 Jan 6. PMID:29412908
Note on publication
Describes the generation and characterization of the antibody 13F7 as well as the construction of an assay detecting blood in food products.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
