Cat. No.
MABL-1803
Application
IP, WB, IHC
Isotype
Engineer antibody
Species Reactivity
Bovine, Cod, Rat, Wolffish, Human, Mouse
Clone No.
A7L6
From
Recombinant Antibody
Specificity
This antibody recognizes and precipitates perlecan, a major heparan-sulfate proteoglycan (HSPG) within all basement membranes and cell surfaces. This macromolecule has the power to store and protect growth factors, and thus plays a major rol in the control of tumour growth.
Alternative Names
Perlecan; Perlecan proteoglycan; Heparan sulfate proteoglycan 2; Schwartz-Jampel syndrome 1; Endorepellin; Basement membrane-specific heparan sulfate proteoglycan core protein; LG3 peptide
UniProt
P98160
Immunogen
The original rat IgG2a version of this antibody was raised by immunizing fisher rats with high molecular mass material from the Engelbreth-Holm-Swarm (EHS) tumor matrix containing laminin, entactin and HSPG, and fusing the spleen cells of these rats with mouse myeloma X63 Ag8.653 cells.
Application Notes
This original IgG2a rat clone can be used to detect and analyze the presence of perlecan. The specificity towards this protein has been confirmed using immunoprecipitation (Couchman, Ljubimov, 1989; PMID: 2477673). The perlecan was confirmed to reside in the basement membrane by means of immunofluorescence using this antibody (Couchman, Ljubimov, 1989; PMID: 2477673). Immunohistochemical staining confirmed that this clone also cross-reacts with other mammalian creatures (Couchman, Ljubimov, 1989; PMID: 2477673). In addition, one group used western blotting and immunohistochemical staining to detect significant differences in weight and distribution of antibodies across heparan sulfate proteoglycans (Tingbø et al., 2006; PMID: 16459119).
Antibody First Published
Couchman JR, Ljubimov AV Mammalian Tissue Distribution of a Large Heparan Sulfate Proteoglycan Detected by Monoclonal Antibodies Matrix. 1989 Aug;9(4):311-21 PMID:2477673
Note on publication
Describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
