Cat. No.
MABL-1807
Application
ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
19D12
From
Recombinant Antibody
Specificity
This antibody is specific for amino acids 'DTHFPICIFCCGCCHRSKCGMCCKT' of human hepcidin- 25 and does not cross react with murine hepcidin-25. Hepcidin is a an iron-regulating peptide hormone made in the liver. Hepcidin is a cationic, cysteine-rich and tightly folded peptide stabilized by 4 disulfide bonds that plays a major role in innate immunity and iron homeostasis. It controls the delivery of iron to blood plasma from intestinal cells absorbing iron, from erythrocyte-recycling macrophages, and from iron- storing hepatocytes. Hepcidin acts by binding to and inactivating the sole cellular iron exporter, ferroprotein, which delivers iron to plasma from all iron-transporting cells.
Alternative Names
HAMP; hHepc; HEPC; LEAP1; LEAP-1; Hepcidin; Liver-expressed antimicrobial peptide 1
UniProt
P81172
Immunogen
The original antibody was generated by immunizing Xenomouse™ IgG2κλ and IgG4κλ with KLH-conjugated human hepcidin peptide 'DTHFPICIFCCGCCHRSKCGMCCKT'.
Application Notes
The original IgG antibody binds human hepcidin-25 with a binding affinity of Kd= 340 pM (PMID： 23945155). This antibody can be used in combination with another anti-hepcidin antibody 19D12 for detection of human hepcidin in human serum using a sandwich ELISA. 19D12 was used as a capture antibody and 23F11 was used for detection. The limit of sensitivity for the assay was 30 pg/mL (PMID： 23945155). This antibody was also used for immunohistochemical analysis of formalin-fixed, paraffin- embedded sections of prostrate cancer tissues (PMID： 25858146). This antibody was also used to block hepcidin in a study to confirm the functionality of hepcidin in breast tumor spheroids. As expected, blockade of hepcidin increased ferroprotein, indicating that hepcidin synthesized by these cells functions in an autocrine fashion and exerts its expected biological activity (PMID： 29695834).
Antibody First Published
Cooke et al. A fully human anti-hepcidin antibody modulates iron metabolism in both mice and nonhuman primates. Blood. 2013 Oct 24;122(17)：3054-61. PMID：23945155
Note on publication
Describes the development of a fully human antibody for use as a potential therapeutic to treat anemia of inflammation (AI) in patients with kidney disease or cancer.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
