Anti-HER2 mAbs [FRP5](MABL-1817)

MABL-1817

agonist, IP, ELISA, FC

Engineer antibody

Human

FRP5

Recombinant Antibody

This antibody recognizes an extracellular epitope of human HER2.

CD340; NEU; HER-2; HER-2/neu; ERBB2; MLN 19; NGL; TKR1; erb-b2 receptor tyrosine kinase 2; Receptor tyrosine-protein kinase erbB-2; Metastatic lymph node gene 19 protein; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2

P04626

The original antibody was generated by immunizing female BALB/c mice with intact SKBR3 human breast tumor cells.

This antibody has been successfully used to immunoprecipitate p185 erbB-2 protein from human SKBR3 breast tumor cell lysates and human erbB-2-transfected mouse HCll R111 cells. Furthermore, the binding of this antibody to surface-expressed HER2 on SKBR3 cells was determined using flow cytometry. Treatment with this antibody led to a rapid increase in the phosphotyrosine content of the erbB-2 protein in various cell lines. The binding of this antibody stimulated the turnover of the erbB-2 protein but did not stimulate DNA synthesis in HCll R111 cells. Therefore, it can be inferred that this antibody acts as a partial ligand agonist (Harwerth et al., 1992; PMID： 1353079). The binding affinity of this antibody for human HER2 was determined using ELISA (Wels et al., 1992; PMID： 1369487). The scFv version of this antibody was used in the generation of scFv(FRP5)-ETA, an antibody-exotoxin recombinant protein and tested for its anti-tumor activity (Harwerth et al., 1993; PMID： 7903153). ScFv(FRP5)-ETA displayed potent antitumoral activity in vitro against a wide range of established and primary human tumor cells, including breast and ovarian carcinomas, squamous cell carcinomas and prostate carcinomas. scFv(FRP5)- ETA also effectively inhibited the in vivo growth of human tumor xenografts and murine and rat tumor cells stably transfected with human c-erbB2 constructs (von Minckwitz et al., 2005; PMID： 16168106).

Harwerth et al. Monoclonal antibodies against the extracellular domain of the erbB-2 receptor function as partial ligand agonists. J Biol Chem. 1992 Jul 25;267(21)：15160-7. PMID：1353079

The original artucle describes the generation and characterization of four antibodies against extracellular domain of human HER2.

100 μg Purified antibody.

1 mg/ml.

Protein A affinity purified

PBS with 0.02% Proclin 300.

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.