Cat. No.
MABL-1818
Application
in vitro, in vivo, inhibit, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
HerG1M
From
Recombinant Antibody
Specificity
This antibody is specific for human Her2/neu.
Alternative Names
CD340; Receptor tyrosine-protein kinase erbB-2; Metastatic lymph node gene 19 protein; MLN 19; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2; p185erbB2
UniProt
P04626
Immunogen
The original antibody was selected from a humanized VH single domain antibody gene library by screening against Her2/neu antigen.
Application Notes
The binding specificity of this humanized single-domain antibody (sdAb; VHH) was tested in an ELISA. This antibody was used to generate an anti-Her2/neu and anti-human Ig bi-specific construct together with Ab04651 [C3]. The bi-specific construct was tested in vitro on BT474 breast cancer cells using a CCK8 assay and demonstrated a significant inhibitory effect on the growth of the breast cancer cells (approximately 50 times more effective than Herceptin). The bi-specific construct also exhibited a significantly prolonged stability in vivo in mice compared to the sdAb version of the antibody (CN110964113B).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
