Cat. No.
MABL-1828
Application
IP, stimulatory
Isotype
Engineer antibody
Species Reactivity
Bovine, Rabbit, Human
Clone No.
IR 83-16
From
Recombinant Antibody
Specificity
This antibody reacts with human INSR, recognising an epitope on the alpha subunit (amino acids 191-290). It does not cross-react with rat insulin receptors, and does cross-react with rabbit, bovine, porcine and ovine receptors.
Alternative Names
CD220; human insulin receptor; insulin receptor; HHF5; IR; 83 16
UniProt
P06213
Immunogen
This antibody was raised by immunising BALB/c mice with IM-9 lymphocytes, and boosting with purified human placental insulin receptor prior to fusion.
Application Notes
This antibody has been used in immunoprecipitation assays, with radiolabelled human insulin receptor (hINSR) and hINSR-radiolabelled insulin complexes (Soos et al, 1986). This antibody has been shown to enhance insulin binding to IM-9 cells (Soos et al, 1986).
Antibody First Published
Soos et al. Monoclonal antibodies reacting with multiple epitopes on the human insulin receptor. Biochem J. 1986 Apr 1; 235(1)： 199–208. PMID：2427071
Note on publication
Describes the original generation of this antibody, and the characterisation of its specificity.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
