Cat. No.
MABL-1829
Application
IB, inhibitory, IP
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
IR 83-22
From
Recombinant Antibody
Specificity
This antibody is specific for human INSR, reacting with an epitope in the alpha subunit (amino acids 485-592). It does not cross-react with rabbit, rat or mouse insulin receptors, and displays very weak cross-reactivity with bovine, porcine and ovine receptors.
Alternative Names
CD220; human insulin receptor; insulin receptor; HHF5; IR; 83 22
UniProt
P06213
Immunogen
This antibody was raised by immunising BALB/c mice with IM-9 lymphocytes, and boosting with purified human placental insulin receptor prior to fusion.
Application Notes
This antibody has been used in immunoprecipitation assays, with both radiolabelled hINSR and hINSR-radiolabelled insulin complexes (Soos et al, 1986). It has also been used in immunoblot analysis with purified hINSR (Soos et al, 1986), revealing that it binds to the receptor’s alpha subunit. This antibody has been shown to cause >80% inhibition of insulin binding to IM-9 cells (Soos et al, 1986), and behaves as a surrogate agonist.
Antibody First Published
Soos et al. Monoclonal antibodies reacting with multiple epitopes on the human insulin receptor. Biochem J. 1986 Apr 1; 235(1)： 199–208. PMID：2427071
Note on publication
Describes the original generation of this antibody, and the characterisation of its specificity.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
