Cat. No.
MABL-1839
Application
in vivo, WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
1F7
From
Recombinant Antibody
Specificity
The anti-idiotypic antibody recognizes antibodies that react to different components of the HIV virus, such as gp120, p24. The antibody does not react with antibodies derived from SLE and B cell lymphoma.
Alternative Names
ATCC No. HB 11286; anti-idiotypic antibody
UniProt
Immunogen
The original antibody was generated by immunizing BALB/c mice with pooled human polyclonal HIVIG.
Application Notes
The HIV-1 antigen capture ELISA was used to test the binding of human monoclonal anti-HIV-1 to the antibody. The antibody bound to anti-p24 antibodies and anti-gp120 antibodies. Western blot analysis confirmed that the antibody reacts with human antibodies of different HIV-1 antigen specificities (Wang et al., 1992; PMID: 1378015). SHIV-infected macaques were immunized with the antibody. Significant increases in breadth and potency of HIV-1 neutralizing antibody titers to one or more virus strains were detected in all three of the 1F7 immunized macaques (US6057421A).
Antibody First Published
Müller et al. Generation and specificity of monoclonal anti-idiotypic antibodies against human HIV-specific antibodies. I. Cross-reacting idiotopes are expressed in subpopulations of HIV-infected individuals. J Immunol. 1991 Aug 1;147(3):933-41. PMID:1861082
Note on publication
The paper describes the generation and characterization of the anti-idiotypic antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
