Cat. No.
MABL-1851
Application
WB, ELISA, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
BB7.2
From
Recombinant Antibody
Specificity
This antibody binds the carboxy terminus of the α2-helix of class I MHC molecule and a turn on one of the underlying β-strands.
Alternative Names
HLA-A; Human leukocyte antigen A; HLA class I histocompatibility antigen; A-2 alpha chain; MHC class I antigen A-2 HLA-A; HLA class I histocompatibility antigen A-28 alpha chain; HLA class I histocompatibility antigen; A-28 alpha chain; MHC class I antigen A-28
UniProt
P04439
Immunogen
The original antibody was generated by immunizing BALB/c mice with papain solubilized HLA- A2 and B7 antigens.
Application Notes
The binding specificity of BB7.2 towards HLA-A2 was assessed using complement- mediated cytotoxicity and cellular radio binding assay. This antibody is cytotoxic for peripheral blood leukocytes and lymphoid cell lines. The original IgG2b version of this antibody binds HLA-A2 positive cells with a binding or avidity constant of about 5 x 108 liter/mol (PMID:7035415). This antibody was used to detect HLA-A*0201-PR1 complexes using western blot and ELISA (PMID: 17493346). This antibody was also used to detect HLA-A2 complexed with pol, gag, and EBV peptides using ELISA (PMID:8562505). This antibody was used to screen human leucocyte antigen (HLA)-A2 haplotype-positive HBV patients using flow cytometry (PMID: 16108766). This antibody was also used for the immunofluorescent staining of HLA-A2.1 molecules on the cell surface of human processing defective cell line (T2) (PMID:7684681). This antibody was used in a study involving the expression of HLA class molecules in head and neck squamous cell carcinoma (HNSCC) specimens by immunohistochemistry (PMID: 9328140).
Antibody First Published
Parham et al. Partial purification and some properties of BB7.2. A cytotoxic monoclonal antibody with specificity for HLA-A2 and a variant of HLA-A28. Hum Immunol. 1981 Dec;3(4):277-99. PMID:7035415
Note on publication
Describes the generation and purification of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
