Cat. No.
MABL-1852
Application
functional assay, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
MBW1
From
Recombinant Antibody
Specificity
The antibody specifically binds human HLA-A2 and does not cross react with A28, Aw68, or Aw69 antigens.
Alternative Names
HLA-A; Human leukocyte antigen A; HLA class I histocompatibility antigen; A-2 alpha chain; MHC class I antigen A-2 HLA-A; HLA class I histocompatibility antigen A-28 alpha chain; HLA class I histocompatibility antigen; A-28 alpha chain; MHC class I antigen A-28
UniProt
P04439
Immunogen
The original antibody was generated from the peripheral blood lymphocytes obtained from a multiparous volunteer 1 week postpartum were transformed in vitro by Epstein-Barr virus and electro fused with the heterohybridoma fusion partner, K6H6/B5. The screening for specific clone was done using micro lymphocytotoxicity assay.
Application Notes
This antibody specifically reacts with human HLA-A2 and does not cross react with A28, Aw68, or Aw69 antigens (PMID: 2160447). The reactivity of this antibody to human HLA-A2 was determined using ELISA. The reactivity of this antibody towards HLA-A2 was abolished by substitutions in the a1 domain, specifically the substitutions of alanine for glutamic acid at position 58 (E58A) and either a valine or arginine substitution for glycine at position 62 (G62V or G62R). The reactivity of this antibody to surface bound HLA- A*0201 was determined using complement-dependent cytotoxicity (CDC) assay (PMID: 9862700).
Antibody First Published
Wallace et al. Generation of a human hybridoma producing a pure anti-HLA- A2 monoclonal antibody. Hum Immunol. 1990 May;28(1):65-9. PMID:2160447
Note on publication
Describes the generation and characterization of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
