Cat. No.
MABL-1855
Application
FC, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
3PF12
From
Recombinant Antibody
Specificity
Amino acid residues 142-145 (TTKH) of the alpha2 domain of the heavy chain of the HLA class I molecules are most likely to be involved in the formation of the epitope recognised by the scfv.
Alternative Names
Human Leukocyte antigen; HLA-A2; HLA-A28; HLA A2; HLA A28
UniProt
Immunogen
A 57-year-old female presented with post-transfusion purpura 5 days following a blood transfusion. Serological studies with the sera of this patient identified the presence of anti-HLA-A2. A 50-ml EDTA anti-coagulated blood sample was obtained from the patient in the acute phase of her PTP, and mononuclear cells were isolated from the blood sample by layering onto a Ficoll gradient. An oligo dT primer was used to direct first strand cDNA synthesis and IgG encoded VH was amplified and an scFv library was constructed. Phage particles expressing anti-HLA A2/A28 scFvs were selected by panning.
Application Notes
ScFv 3PF12 has been used in immunofluorescence and flow cytometry experiments to test antibody binding to HLA-A2 positive lymphocytes and platelets (Watkins 2000). The scFv has been tested in in vitro assays and have been shown to inhibit target cell killing (Watkins 2003).
Antibody First Published
Watkins et al. The isolation and characterisation of human monoclonal HLA- A2 antibodies from an immune V gene phage display library Tissue Antigens 2000:55: 219–228 PMID:10777097
Note on publication
Describes the isolation of scFvs against HLA-A2/A28 by V gene phage display.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
