Cat. No.
MABL-1864
Application
Immunoperoxidase staining, IP, RIA, WB, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
Lym-1
From
Recombinant Antibody
Specificity
This antibody has high affinity for a discontinuous epitope on the β subunit of the human HLA- DR10 surface membrane antigen of malignant B cells, which is not expressed on normal lymphocytes. HLA- DR10 (DR10) is a HLA-DR serotype that recognizes the DRB1*1001 gene product.
Alternative Names
CD74; Beta chain HLA-DR; HLA-DR β-chain; β-chain; HLA class II histocompatibility antigen gamma chain; HLA-DR antigens-associated invariant chain; Ia antigen-associated invariant chain; Ii; p33
UniProt
P04233
Immunogen
The original antibody was generated by immunizing mice with tumor cell nuclei preparations as immunogens.
Application Notes
This antibody could recognize cell lines of B-cell lineage but not cells of T-cell, myeloid, or erythroid derivation in a live cell radioimmunoassay. Flow cytometric analysis of 22 lymphoma biopsies showed that the majority of B-cell tumors were LYM-1 positive. Approximately 40% of B-cell chronic lymphocytic leukemias were found to be positive with Lym-1. The binding of this antibody to peripheral blot lymphocytes was assessed using indirect immunofluorescence staining. Immunoperoxidase staining of nonlymphoid human organs and tissues revealed weak reactivity of Lym-1. This antibody was also capable of immunoprecipitating HLA-DR10 antigen from Raji cells and ARH-77 myeloma cells. (PMID: 3542194). This antibody also was used for immunohistochemical staining of advanced B-cell NHL tissues from patients (PMID: 10809213). This antibody can be used to identify the HLA-DR variant HLA-DR10 in a western blot (PMID: 8917632). LYM-1 chimeric antigen receptor T cells were demonstrated to exhibit potent anti-tumor effects against B-cell lymphoma (PMID: 29261129).
Antibody First Published
Epstein et al. Two new monoclonal antibodies, Lym-1 and Lym-2, reactive with human B-lymphocytes and derived tumors, with immunodiagnostic and immunotherapeutic potential. Cancer Res. 1987 Feb 1;47(3):830-40. PMID:3542194
Note on publication
Describes the immunodiagnostic and immunotherapeutic potential of two monoclonal antibodies reactive with tumor derived human B cell lymphocytes.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
