Anti-HLA-DR mAbs [L243](MABL-1863)

MABL-1863

ICC, in vivo, IP, WB, Block, ELISA, FC, IF, IHC

Engineer antibody

Dog, Human

L243

Recombinant Antibody

This antibody specifically reacts with the HLA-DR antigen and does not cross-react with HLA-DP or HLA-DQ. It binds to a conformational epitope on HLA-DRα, which requires the proper folding of the αβ heterodimer. HLA-DR, a major histocompatibility complex (MHC) class II cell surface receptor, is encoded by the human leukocyte antigen complex located on chromosome 6 at region 6p21.31. HLA-DR plays a key role in autoimmune conditions, influencing both disease susceptibility and resistance. Its primary function is to present peptide antigens, including those of foreign origin, to the immune system. This process helps elicit or suppress T-helper cell responses, which eventually lead to the production of antibodies against the specific peptide antigen.

CD74; HLA class II histocompatibility antigen gamma chain; HLA-DR antigens-associated invariant chain; Ia antigen-associated invariant chain; Ii; p33

P04233

The original antibody was generated by immunizing mice with the human B cell line RPMI 8866.

This antibody can be used for the immunoprecipitation of HLA-DR and MHC class-II peptides (Bonnefoy et al., 1988; PMID: 2961843). This antibody selectively inhibited the in vitro proliferation of Epstein-Barr virus-transformed lymphoblastoid cell lines (Kabelitz and Janssen, 1989; PMID: 2467753). This antibody can also be used for the immunocytochemical and immunohistochemical analysis of frozen sections of necropsy brain tissue to identify HLA-DR-expressing cells (Esiri et al., 1991; PMID: 1864982). This antibody was used in the detection of MHC class II protein in a western blot (Esser et al., 2001; PMID: 11390619). This antibody was used in a flow cytometric assay to detect the decrease in HLA-DR expression on monocytes, which is an indicator of immunosuppression in patients with sepsis and is correlated with an increased risk of secondary infection and mortality (Quadrini et al., 2021; PMID: 33432735). Alexa 568- and FITC-labeled versions of this antibody were used in the immunofluorescent staining of renal microvascular endothelial cells (RMEC) or human kidney tissue (Muczynski et al., 2003; PMID: 12707403). This antibody was assessed in a pilot study using a canine lymphoma model. In vitro studies showed that this antibody binds to both healthy and lymphoma canine lymphocytes, inducing apoptosis in canine non-Hodgkin lymphoma cells. In vivo studies confirmed the antibody's safety in healthy and lymphoma-affected dogs, as well as its binding activity in lymphoma-affected lymph nodes. Preliminary results indicated transient disease stabilization in a subset of dogs with advanced lymphoma (Stein et al., 2011; PMID: 21133722).

Lampson et al. Two populations of Ia-like molecules on a human B cell line. J Immunol. 1980 Jul;125(1):293-9. PMID:6966655

Describes the generation of two antibodies against Ia-like molecules on the human B cell line Raji.

100 μg Purified antibody.

1 mg/ml.

Protein A affinity purified

PBS with 0.02% Proclin 300.

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.