Cat. No.
MABL-1867
Application
WB, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
9Y5G6
From
Recombinant Antibody
Specificity
This antibody specifically binds the amino acid sequence 'SKEGDGGIMSVRSLSED' of the human HLA-G5 and HLA-G6. HLA-G5 and HLA-G6 are secreted forms of HLA-G that are non-classical major histocompatibility class Ib molecules involved in immune regulatory processes at the maternal-fetal interface. HLA-G expression has been reported in numerous types of cancer, always associated with more advanced stage and aggressive development of the tumor.
Alternative Names
HLAG; HLA-6.0; HLA-G5; HLAG-6; HLA class I histocompatibility antigen alpha chain G; HLA G antigen; MHC class I antigen G; HLA-G1sol; HLA-G2sol
UniProt
P17693
Immunogen
The original antibody was generated by immunizing BALB/c mice with synthetic human HLA-G molecule heavy chain amino acid sequence '229SKEGDGGIMSVRSLSED319'.
Application Notes
This antibody can bind human HLA-G peptide 'KEGDGGIMSVRSLSED' in an ELISA with a binding affinity of Ka= 1.5 X 10^(11) L/mol. This antibody was capable of detecting G5 and G6 isoforms of human HLA-G in a western blot and does not cross react with any other isoforms of HLA-G. This antibody can also be used for the immunohistochemical detection of HLA-G5 and HLA-G6 molecules in different gastric cancer tissues using immunohistochemistry (CN113045656).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
