Cat. No.
MABL-1868
Application
ELISA, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
mem-G/9
From
Recombinant Antibody
Specificity
This antibody binds an epitope in the alpha 1 domain of human HLA-G. It binds the native form of human HLA-G1 on the cell surface as well as with soluble HLA-G5 isoform in its beta2-microglobulin associated form. It is also reported to cross react with HLA-G3.
Alternative Names
HLAG; HLA-6.0; HLA-G5; HLAG-6; HLA class I histocompatibility antigen alpha chain G; HLA G antigen; MHC class I antigen G; human leukocyte antigen
UniProt
P17693
Immunogen
The original antibody was generated by immunizing mice with native, properly folded, beta-2- microglubulin (b2m)-associated HLA-G.
Application Notes
This antibody is not suitable for detecting denatured human HLA-G protein in a western blot. This antibody can be used for the detection of soluble HLA-G in a sample using ELISA (PMID: 10902607). This antibody can effectively detect HLA-G and HLA-G3 on K653-HLA-G1, K562-G3 transductants, M8-HLA-G1 and JEG-3 cells in a flow cytometric analysis (PMID: 12590976; 22738368). This antibody was also used for studying the expression of HLA-G on the surface of JEG-3 cells using immunofluorescence staining (PMID: 22738368). This antibody can be used for immunohistochemical analysis of cryopreserved serial sections of trophoblast tissue. This antibody is not suitable for immunohistochemical staining of paraffin sections (PMID: 12590976). This antibody was also reported to be used in isolation of HLA-G+ cells for prenatal screening (PMID: 35498932).
Antibody First Published
Fournel et al. Comparative reactivity of different HLA-G monoclonal antibodies to soluble HLA-G molecules. Tissue Antigens. 2000 Jun;55(6):510-8. PMID:10902607
Note on publication
This paper evaluated the ability of different HLA-G antibodies to react with soluble HLA-G in an ELISA.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
