Cat. No.
MABL-1869
Application
WB, ELISA, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
YWHG-5
From
Recombinant Antibody
Specificity
This antibody specifically binds the amino acid sequence 'EGLPEPLMLRWSKEGDGGIMS' in the α3 structural domain of the human HLA-G5 and HLA-G6. HLA-G5 and HLA-G6 are secreted forms of HLA-G that are non-classical major histocompatibility class Ib molecules involved in immune regulatory processes at the maternal-fetal interface. HLA-G expression has been reported in numerous types of cancer, always associated with more advanced stage and aggressive development of the tumor.
Alternative Names
HLAG; HLA-6.0; HLA class I histocompatibility antigen alpha chain G; HLA G antigen; MHC class I antigen G
UniProt
P17693
Immunogen
The original antibody was generated by immunizing BALB/c mice with synthetic human HLA-G molecule heavy chain α3 structural domain and HLA-G5/6 molecule specific transmembrane region amino acid sequence 'EGLPEPLMLRWSKEGDGGIMS'.
Application Notes
This antibody can bind human HLA-G peptide 'EGLPEPLMLRWSKEGDGGIMS' in an ELISA with a binding affinity of Ka= 1.01 X 10^(9) L/mol. This antibody was capable of detecting G5 and G6 isoforms of human HLA-G in a western blot and does not cross react with any other isoforms of HLA-G. This antibody was also used in the detection of soluble HLA-G5 and HLA-G6 using intracellular flow cytometry of K562-HLA-G5 and K562-HLA-G6 cells. This antibody can also be used for the immunohistochemical staining of HLA-G5/6 molecular expression in gastric cancer tissue using immunohistochemistry (CN114605543).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
