Cat. No.
MABL-1873
Application
in vivo, WB, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Rat
Clone No.
2G7
From
Recombinant Antibody
Specificity
This antibody is specific for amino acids 53-63 of HMGB1.
Alternative Names
Hmg-1; Hmg1; Amphoterin; Heparin-binding protein p30; High mobility group protein 1; High mobility group protein B1
UniProt
P63159
Immunogen
The original antibody was raised by immunizing BALB/c mice with recombinant CBP-Rat HMGB1.
Application Notes
The original antibody was used for an ELISA on recombinant rat HMGB1. This antibody was used for a western blot on recombinant rat HMGB1. The epitope of this antibody was determined using peptide binding. Mice were subjected to a cecal ligation and puncture, after which this antibody was administered. It showed an increased survival rate in these septic mice (US7632500B2). This antibody was used for immunofluorescence on RAW 264.7 cells, to study intracellular HMGB1. This antibody was used for an ELISPOT on RAW 264.7, C57 and HMC-12 cells (Wähämaa et al., 2007; PMID:16980509). This antibody was administered to mice subjected to ACLT, the antibody was able to protect cartilage (Aulin et al., 2020; PMID:31982563). This antibody was tested in two murine arthritis models, it showed a decrease in general disease severity (Schierbeck et al., 2011; PMID:21666956). This antibody was used in vivo on SOD1G93A transgenic mice, to test the effect of this antibody on ALS, the antibody did not increase survival time but it did improve early motor deficits and reduced inflammation (Lee et al., 2019; PMID:30782181).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
