Cat. No.
MABL-1881
Application
FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
HIC1-2B4
From
Recombinant Antibody
Specificity
This antibody binds the HPi2 marker on the surface of human pancreatic enriched islet cells.
Alternative Names
Human Pancreas Endocrine Islet Cell Marker 2; Human pancreatic marker; Endocrine pan-islet cell marker
UniProt
Immunogen
The original antibody was generated by immunizing BALB/c mice with endocrine cells of the islets of Langerhans.
Application Notes
This antibody binds distinct surface molecules expressed on endocrine cells and have been validated for a number of experimental methods including immunohistochemistry and live cell sorting by flow cytometry. This antibody can be used individually or in combination with others to isolate populations of α, β, exocrine, or duct cells from primary human pancreas by FACS and to characterize the detailed cell composition of human islet preparations (PMID: 19383399, 27641999). This antibody was used in combination with HIC3-2D12 to identify and isolate major subtypes of the human islet cells including α- and β-cells and the purity levels of the isolated cells was determined using immunofluorescence staining (PMID: 31780397). Humanized version of this antibody has also been reported (PMID: 29758224). Chimeric antigen receptor (CAR) with scFv form of this antibody was used in the novel Tregs-based antigen-specific Type 1 Diabetes (T1D) immunotherapy (PMID: 33068914).
Antibody First Published
Dorell et al. Isolation of major pancreatic cell types and long-term culture- initiating cells using novel human surface markers. Stem Cell Res. (2008); 1(3):183-94. PMID:19383399
Note on publication
Describes the development of a panel of antibodies for the isolation and study of major cell types of the human pancreas.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
