Cat. No.
MABL-1883
Application
IP, neutralize, ELISA, FC, IF
Isotype
Engineer antibody
Species Reactivity
Human papillomavirus type 16
Clone No.
H16.V5
From
Recombinant Antibody
Specificity
This antibody recognizes a surface confirmation epitope on the intact HPV-16 L1 virus like particles. This antibody is known to recognize the FG and HI loops, which are immunodominant in the humoral response against the HPV16 major capsid protein.
Alternative Names
L1; Major capsid protein L1
UniProt
P03101
Immunogen
The original antibody was generated by immunizing mice with recombinant baculovirus- induced HPV-6 L1 VLPs that were purified from infected Sf9 cells by CsCl-banding.
Application Notes
The specificity of this antibody against intact and disrupted virus-like particles (VLPs) of HPV-6, HPV-16, HPV-11, and HPV-18 was determined using ELISA (PMID: 8874508). This antibody was used as a capture antibody to test human serum IgG reactive with HPV-16 capsids (PMID: 8896492). This antibody was also reported to block HPV-16 VLP-induced agglutination of mouse RBC (PMID: 8806551). This antibody is capable of neutralizing by inhibiting HPV-16 binding to cell surfaces (PMID: 9223527). This antibody was also used to characterize a major neutralizing epitope on the HPV-16 type virus (PMID: 10233949). Sequences at both ends of the long FG loop (amino acids 260 to 290) were required for H16.V5 reactivity (PMID: 14557648). H16.V5 is often used for the assessment of the integrity and antigenicity of VLPs in vaccine products (PMID: 17012876). This antibody, when conjugated with magnetic beads, can also be used to immunoprecipitate HPV16 VLP and PsV out of cell lysates (PMID: 22927980). The binding to the surfaces of CaSki cells was detected by indirect immunofluorescence staining with H16.V5 and flow cytometry. The ability of gradient-purified HPV16 L1 to bind to the surface of SiHa cells at 4°C was detected by flow cytometric staining with the H16.V5 monoclonal antibody (PMID: 15890912).
Antibody First Published
Christensen et al. Monoclonal antibodies to HPV-6 L1 virus-like particles identify conformational and linear neutralizing epitopes on HPV-11 in addition to type-specific epitopes on HPV-6. Virology. 1996 Oct 15;224(2):477-86. PMID:8874508
Note on publication
Describes the generation of antibodies against the HPV-6 L1 virus-like particles (VLPs) and identifies whether these antibodies can be used in the neutralization of HPV11 infection.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
