Cat. No.
MABL-1895
Application
IP
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
W6/1
From
Recombinant Antibody
Specificity
W6/1 binds to A1 and A2 erythrocytes and does not bind to B or O erythrocytes (detects a polymorphic determinant of erythrocytes correlating with blood group A). When saturating amounts of secondary antibody are used, binding of W6/1 is 5 times as great on type A1 than A2.
Alternative Names
UniProt
Immunogen
W6/1 was prepared by immunizing BALB/c mice with membrane from human tonsil cells (present as a result of contaminating erythrocyte membrane in the tonsil leucocyte preparation).
Application Notes
W6/1 antibody can be used as an erythrocyte cell type marker and can cause hemogglutination of A-type erythrocytes.
Antibody First Published
Barnstable et al Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens-new tools for genetic analysis. Cell 14:9-20 1978 PMID:667938
Note on publication
Describes the production of W6/1 antibody and determination of its binding specificity.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
