Cat. No.
MABL-1894
Application
crystallization, in vitro, SPR, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
scFv-C4
From
Recombinant Antibody
Specificity
The antibody is specific for huntingtin. It binds to the first 17 residues of huntingtin [HTT(1- 17)].
Alternative Names
C4; HTT; Huntingtin; HD protein; Huntington disease protein
UniProt
P42858
Immunogen
The original antibody was isolated screening a naïve human single-chain Fv antibody phage- display library against HTT(1-17).
Application Notes
The original format of the antibody could inhibit the aggregation of mHTT-exon1 protein fragments in vitro. The crystal structure of the antibody in complex with HTT(1-17) was determined (De Genst et al., 2015; PMID: 25861763). The specificity of the antibody was confirmed by ELISA analysis. The original format of the antibody-targeted the HD-Q103-myc antigen to cell nuclei in COS-7 cells by immunofluorescence microscopy. The binding affinity of the antibody to HTT(1-17) was measured by surface plasmon resonance (Kd= 7.9 nM) (Lecerf et al., 2001; PMID: 11296304). The antibody selectively bound diffuse htt, but not aggregated htt, as shown by immunofluorescence analysis. Immunoprecipitation experiments in stably transfected, intrabody-expressing STHdhQ111 cells demonstrated that the antibody bound a small fraction of wild-type and mutant endogenous full-length htt (Miller et al., 2005; PMID: 15837560).
Antibody First Published
Lecerf et al., Human single-chain Fv intrabodies counteract in situ huntingtin aggregation in cellular models of Huntington's disease. Proc Natl Acad Sci U S A. 2001 Apr 10;98(8):4764-9. doi: 10.1073/pnas.071058398. PMID:11296304
Note on publication
The original paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
