Cat. No.
MABL-1898
Application
RIA, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
AB4
From
Recombinant Antibody
Specificity
Alternative Names
hCG; Human chorionic gonadotropin; Choriogonadotropin subunit beta
UniProt
P01233
Immunogen
Human chorionic gonadotropin.
Application Notes
The antibody binds specifically to hCG, a hormone produced by a part of the placenta, the syncytiotrophoblast, following implantation. The hormone has an important role during pregnancy, as it interacts with the LHCG receptor in the ovaries to prevent the degeneration of the corpus luteum, and thereby allows the secretion of progesterone during the first trimester. hCG is a heterodimeric glycoprotein, composed of an α and a β domain, of which the α is identical to that of LH and FSH, while the β is unique to hCG. The monoclonal antibody AB4 is specific for the β-subunit, and can be used in the detection of the hormone in pregnancy tests or in the diagnosis of uterine cancer.
Antibody First Published
de Haard HJ, Kazemier B, van der Bent A, Oudshoorn P, Boender P, van Gemen B, Arends JW, Hoogenboom HR. Absolute conservation of residue 6 of immunoglobulin heavy chain variable regions of class IIA is required for correct folding. Protein Eng. 1998 Dec;11(12):1267-76. PMID:9930677
Note on publication
Describes the generation of scFvs from murine monoclonal antibodies, and the effect of several amino acid mutations on the immunoglobulin fold and antigen binding affinity.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
