Cat. No.
MABL-1914
Application
IF
Isotype
Engineer antibody
Species Reactivity
Caenorhabditis elegans
Clone No.
KT13
From
Recombinant Antibody
Specificity
This antibody recognizes fibrous structures in seam cells of hypodermis of Caenorhabditis elegans that are detectable only beginning from the pretzel stage. Caenorhabditis elegans is a transparent nematode that lives in temperate soil environments.
Alternative Names
Seam cells
UniProt
Immunogen
The original antibody was generated by immunizing BALB/c mice with subtracted embryonic extract. Subtracted extracts were prepared by using a method called “antigen subtraction” which enriched antigens of low abundance in heterogeneous molecule mixtures by subtracting abundant antigens.
Application Notes
This antibody stained distinct subsets of hypodermal cells at specific periods during embryogenesis. This antibody was also used for staining of the seam cells of a pretzel stage embryo. This antibody recognized KT13 recognized the lateral hypodermis (called seam cells) at the pretzel stage, but not at earlier stages. Seam cells at the pretzel stage have a long rectangular shape, and the this antibody sends signals that were stronger along the edge of the long sides of the rectangle. With higher magnification, The KT13 signal appeared as parallel fibrous structures along the short axis of each seam cell (PMID:18498356).
Antibody First Published
Takeda et al. Efficient production of monoclonal antibodies recognizing specific structures in Caenorhabditis elegans embryos using an antigen subtraction method. Genes Cells. 2008 Jul;13(7):653-65. PMID:18498356
Note on publication
Describes the use of "antigen subtraction" method to enrich antigens of low abundance in heterogeneous molecule mixtures by subtracting abundant antigens. The paper also discloses generation of 35 mAbs against Caenorhabditis elegans that recognize specific structures, including P granules, muscles, the pharynx, and subsets of hypodermal cells; some of the mAbs revealed previously unreported cellular structures.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
