Cat. No.
MABL-1915
Application
FC, IF
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
AF6-120.1.2
From
Recombinant Antibody
Specificity
This antibody is specific for the mouse Mouse I-Ab antigen.
Alternative Names
H-2 class II histocompatibility antigen, A-B alpha chain; H2-Aa; Iaalpha
UniProt
P14434
Immunogen
The antibody was raised against the mouse I-Ab antigen.
Application Notes
Cloned lines of functionally active murine T lymphocytes derived from secondary mixed leukocyte cultures (MLC) were immunostained with this antibody (Lorber et al, 1982; pmid:6804568). Cloned lines of functionally active murine T lymphocytes derived from secondary mixed leukocyte cultures (MLC) were used in flow cytometry using this antibody (Lorber et al, 1982; pmid:6804568). Immunofluorescence was preformed on splenocytes from NOD, NON and CTS mice using this antibody (Ikegami et al, 1988; pmid:3133269). Flow cytometry was preformed using this antibody on TC-1. TC-1 is a malignant, immunogenic cell line of C57BL/6 mouse origin expressing HPV16 E6/E7 antigens (Manning et al, 2008; pmid:17725605). Cells from a p50-/- mouse were used in a flow cytometry ecperiment (Sha et al, 1995; pmid:7834752).
Antibody First Published
Lorber et al. I-A ANTIGENS ON CLONED ALLOREACTIVE MURINE T LYMPHOCYTES ARE ACQUIRED PASSIVELY J Immunol. 1982 Jun;128(6):2798-803. PMID:6804568
Note on publication
Cloned lines of alloreactive cytolytic and amplifier T lymphocytes were examined for expression of I-A antigens.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
