Cat. No.
MABL-1917
Application
therapeutic, WB, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
infectious bursal disease virus
Clone No.
CRAb20
From
Recombinant Antibody
Specificity
This antibody is specific for infectious bursal disease virus (IBDV). IBDV is a highly contagious disease of young chickens and turkeys caused by infectious bursal disease virus (IBDV),[1] characterized by immunosuppression and mortality generally at 3 to 6 weeks of age.
Alternative Names
Infectious bursal disease virus
UniProt
Immunogen
The original anitbody was isolated from antibody libraries derived from splenic mRNA of chickens immunized with an Australian strain of infectious bursal disease virus (IBDV). The antibody was isolated by panning the chiken scFv library against IBDV strain 002/73.
Application Notes
To characterize this antibody an ELISA was preformed on IBDV using a phage expressing the scFv version of this antibody. Furthermore, to assess the ability of this antibody to neutralize IBDV, the scFv version of this antibody was mixed with chicken embryo fibroblasts and the GT101 version of IBDV. This antibody showed the ability to neutralize the virus to a considerable extent (Sapats et al, 2003; pmid：12607101). To investigate high pressure processing as a means to inactivate avian viral pathogens in chicken meat homogenate, the scFv version of this antibody was used for immunohistochemistry on bursae from chicken. Furthermore, ELISA was preformed on the chorioallantoic membrane harvested from chicken eggs using the scFv version of this antibody (Buckow et al, 2017). To improve the recombinant antibody- based serological assay for foot-and-mouth disease virus, a western blot was preformed using the scFv version of this antibody on 3B protein (Muller et al, 2010; pmid：19913020).
Antibody First Published
Sapats et al. Generation of chicken single chain antibody variable fragments (scFv) that differentiate and neutralize infectious bursal disease virus (IBDV). Arch Virol. 2003 Mar;148(3):497-515. PMID:12607101
Note on publication
Phage-displayed recombinant antibody libraries derived from splenic mRNA of chickens immunized with an Australian strain of infectious bursal disease virus (IBDV) were constructed as single chain variable fragments (scFv) by either overlap extension polymerase chain reaction (PCR) or sequential ligation of the individual heavy (V(H)) and light (V(L)) chain variable gene segments.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
