Cat. No.
MABL-1930
Application
ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
10G5-1
From
Recombinant Antibody
Specificity
This antibody binds human isocitrate dehydrogenase 1 (IDH1) having R132H mutation. IDH1 is an enzyme that catalyzes the NADP+-dependent oxidative decarboxylation of isocitrate (D-threo-isocitrate) to 2-ketoglutarate (2-oxoglutarate), which is required by other enzymes such as the phytanoyl-CoA dioxygenase. It plays a critical role in the generation of NADPH, an important cofactor in many biosynthesis pathways. The NADPH produced from isocitrate dehydrogenase 1 is involved in the breakdown of fats for energy, and it also protects cells from potentially harmful molecules called reactive oxygen species. Mutation in IDH1 are found in several genetic conditions and in some types of cancer, including gliomas, cartilaginous tumors, acute myelogenous leukemia, myelodysplastic syndromes, and brain cancer.
Alternative Names
IDH; IDH1; IDPc; Isocitrate dehydrogenase [NADP] cytoplasmic; Isocitrate dehydrogenase 1; Cytosolic NADP-isocitrate dehydrogenase; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase
UniProt
O75874
Immunogen
The original antibody was generated by immunizing BALB/c mice with human IDH-R132H polypeptide (KPIIGHHAYGDQYC) with an N-terminal BSA conjugation.
Application Notes
The binding reactivity of this antibody to R132H mutant of human IDH1 was determined using ELISA. The original mouse IgG antibody binds IDH1 R132H mutant with an affinity of Kd= 5.76 X10 ^(-10) M. This antibody was used in the immunohistochemical analysis for detection of IDH1 R132H protein in cancerous tissues (CN108727500).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
