Cat. No.
MABL-1933
Application
WB, Block, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Baboon, Human
Clone No.
D9D10
From
Recombinant Antibody
Specificity
Binds human interferon-gamma, a dimerized soluble cytokine that is the only member of the type II class of interferons. Cross reacts with baboon IFN gamma.
Alternative Names
Interferon gamma; Immune interferon ; IFN-gamma; IFNG
UniProt
P01579
Immunogen
FPLC-purified natural human IFN-y. Refer to the original article [PMID: 3114127]
Application Notes
This antibody can be used to detect both native and recombinant intereferon gamma (IFNg). It is reported only to bind to the functional, dimeric form of IFNg.
Antibody First Published
Froyen G, Ronsse I, Billiau A. Bacterial expression of a single-chain antibody fragment (SCFV) that neutralizes the biological activity of human interferon-gamma. Mol Immunol. 1993 Jun;30(9):805-12. PMID:8321246
Note on publication
Describes the preparation anti human interferon gamma antibody by D9D10 hybridoma seqencing (VH and VK) and identified using ELISA and SDS-PAGE (for neuralisation of antiviral activity) techniques.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
