Cat. No.
MABL-1952
Application
functional assay, therapeutic, WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
7C10
From
Recombinant Antibody
Specificity
The antibody is specific for human IGF-IR. It does not prevent clone 12BI from binding to the same protein. IGF-IR is a receptor tyrosine kinase which mediates actions of insulin-like growth factor 1 (IGF1). Binds IGF1 with high affinity and IGF2 and insulin (INS) with a lower affinity. The activated IGF1R is involved in cell growth and survival control.
Alternative Names
Insulin-like growth factor 1 receptor; IGF1R; Insulin-like growth factor I receptor; IGF-I receptor; CD221
UniProt
P08069
Immunogen
The original mouse antibody was raised by immunizing mice with recombinant human IGF-IR.
Application Notes
Specificity of this antibody was confirmed by ELISA (WO2009017679). Further, western blot was preformed to test whether the antibody cross reacts with IR protein (WO2009017679). It was also observed that the human version of this antibody was useful for antibody dependent cell cytotoxicity assays; MCF-7 or A549 target cells were incubated with this antibody and human natural killer cells. It was shown that the antibody does increase lysis of these cells (Goetsch et al, 2005; pmid:15386423). Furthermore, it was shown that the human version of this antibody is able to inhibit growth of MDA-MB-231, an oestrogen-independent breast cancer cell line in mice (Pandini et al, 2007; pmid:17451939). The human version of this antibody was also used in immunofluorescence on MCF-7 cells (Broussas et al, 2009; pmid:19165858). Finally, human version of this antibody was utilized in western blot of MCF-7 cells tumors (Broussas et al, 2009; pmid:19165858).
Antibody First Published
Goetsch et al. A recombinant humanized anti-insulin-like growth factor receptor type I antibody (h7C10) enhances the antitumor activity of vinorelbine and anti-epidermal growth factor receptor therapy against human cancer xenografts Int J Cancer. 2005 Jan 10;113(2):316-28. PMID:15386423
Note on publication
Describes the generation of a humanized anti-IGF-IR antibody h7C10 that blocks in vitro IGF-I and IGF-II-induced cell proliferation of MCF-7 breast cancer cells.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
