Cat. No.
MABL-1953
Application
IP;, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human, Cynomolgus Monkey
Clone No.
10H5
From
Recombinant Antibody
Specificity
This antibody binds the extracellular domain of the human and cynomolgus monkey insulin-like growth factor and effective blocks ligand (IGF-I and IGF-II)-receptor (IGF-IR) interaction. This antibody does not bind mouse or rodent IGF-1R.
Alternative Names
CD221; IGF1 receptor; Insulin-like growth factor 1 receptor; type I IGF receptor; Insulin-like growth factor I receptor; IGF-I receptor
UniProt
P08069
Immunogen
The original antibody was generated from BALB/c mice hyperimmunized with human IGF-IR extracellular domain (amino acids 1-902) in Ribi adjuvant. Later on the humanized version was created by grafting the hypervariable regions onto human framework regions.
Application Notes
The orginal humanized IgG1 version of this antibody binds human IGF-1R with an affinity of 1.1 nM in an ELISA. The binding specificity was also comfirmed using fluorescence activated cell sorting of IGF-IR-expressing MCF7 cells. This antibody was also used for the immunoprecipitaion of MCF7 cells. The original antibody inhibits IGF-IR-mediated signaling by blocking IGF-I and IGF-II binding and by inducing cell surface receptor downregulation via internalization and degradation, with the extracellular and intracellular domains of IGF-IR being differentially affected by the proteasomal and lysosomal inhibitors. In vitro, h10H5 exhibits antiproliferative effects on cancer cell lines. In vivo, h10H5 shows single-agent antitumor efficacy in human SK-N-AS neuroblastoma and SW527 breast cancer xenograft models and even greater efficacy in combination with the chemotherapeutic agent docetaxel or an anti–vascular endothelial growth factor antibody (PMID:18790743).
Antibody First Published
Shang et al. Antixenograft tumor activity of a humanized anti-insulin-like growth factor-I receptor monoclonal antibody is associated with decreased AKT activation and glucose uptake. Mol Cancer Ther. 2008 Sep;7(9):2599-608. PMID:18790743
Note on publication
Describes the generation, in vitro and in vivo characterization of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
