Cat. No.
MABL-1954
Application
IP, stimulatory
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
IGFR 16-13
From
Recombinant Antibody
Specificity
This antibody is specific for the human insulin-like growth factor 1 receptor (IGF1R). It recognises the extracellular portion of the receptor, specifically a conformation-dependent epitope in the alpha subunit (amino acids 62-184). It does not cross-react with rat or mouse IGF1R or human insulin receptors in co-precipitation or immunodepletion assays.
Alternative Names
CD221; IGF1 receptor; Insulin-like growth factor 1 receptor; type I IGF receptor; Insulin-like growth factor I receptor; IGF-I receptor; 6 13
UniProt
P08069
Immunogen
This antibody was raised by immunising BALB/c mice with transfected mouse fibroblasts expressing high levels of human IGF1R (IGF1R/3T3).
Application Notes
The ability of this antibody to bind human IGF1R has been confirmed in IGF1 binding assays using both IG1FR-overexpressing IGF1R/3T3 cells and soluble receptors (Soos et al, 1992). This antibody efficiently precipitates IGF1R solubilized from IGF1R/3T3 cells and receptor-IGF1 complexes (Soos et al, 1992). When immobilised, this antibody has been shown to remove 90% of all soluble IGF1R in immunodepletion experiments (Soos et al, 1992). It has also been used to capture IGF1R N-terminal fragments as part of a ligand plate binding assay (Molina et al, 2000). This antibody strongly stimulates both IGF1 and insulin binding to IGF1R/3T3 cells, and potentiates insulin stimulation of DNA synthesis (Soos et al, 1992).
Antibody First Published
Soos et al. A panel of monoclonal antibodies for the type I insulin-like growth factor receptor. Epitope mapping, effects on ligand binding, and biological activity. J Biol Chem. 1992 Jun 25;267(18):12955-63. PMID:1377676
Note on publication
Describes the original generation of this antibody, and the characterisation of its specificity.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
