Cat. No.
MABL-1968
Application
IP, WB
Isotype
Engineer antibody
Species Reactivity
rhesus macaque, Human, Cynomolgus Monkey
Clone No.
NH3/130.5.2
From
Recombinant Antibody
Specificity
This antibody recognises human IgG. The antibody cross-reacts with rhesus macaque IgG1, cynomolgus monkey IgG1 and cynomolgus monkey IgG4.
Alternative Names
immunoglobulin G; human immunoglobulin; NH3/130
UniProt
Immunogen
This antibody was raised by immunising Balb/c mice with whole IgG fraction and subsequent hybridoma cells formation.
Application Notes
This antibody was shown to have a high avidity to human immunoglobulin G having a high titre, equal to a corresponding rabbit polyclonal antibody, when tested against anti-D sensitised cells (Downie et al., 1983). NH3/130.5.2 detects human IgG samples transferred from the SDS polyacrylamide gel via Western blot under non-reducing conditions (Thorpe et al., 1984). Thorne et al. (1984) mentions that this antibody was also successful in immunoprecipitation in agar or solution (M. Spitz, unpublished results). Further, the antibody was capable of detecting IgGs via Western blot from native gels run under acidic conditions (Thorne et al., 1984). Finally, the antibody cross-reacted with rhesus macaque IgG1, cynomolgus monkey IgG1 and cynomolgus monkey IgG4 as shown by ELISA analysis.
Antibody First Published
Downie et al. The use of monoclonal antibodies to human IgG in blood transfusion serology. Biotest Bulletin 4: 348-352 (1983)
Note on publication
This article describes the generation and characterisation of the NH3/130.5.2 antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
