Cat. No.
MABL-1969
Application
HA, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
RF61
From
Recombinant Antibody
Specificity
This antibody is specific for the C-terminal (Cγ3) end of the Fc region of IgG1, -2, and -3, with ~10-fold lower affinity for IgG4. This antibody's binding to IgG Fc is non-inhibitable by S. aureus protein A (SpA), which binds IgG Fc at the Cγ2–Cγ3 interface; this antibody's epitope is almost as far as physically possible from the antigen-combining site of IgG and is also remote from the functional sites responsible for FcγR or C1q binding (in Cγ2) and from the N-linked carbohydrate, also in Cγ2.
Alternative Names
IgG1; IgG2; IgG3; IgG4; Immunoglobulin G; Immunoglobulin gamma-1; Immunoglobulin G subclass 1; Immunoglobulin gamma-2; Immunoglobulin G subclass 2; Immunoglobulin gamma-3; Immunoglobulin G subclass 3; Immunoglobulin gamma-4; Immunoglobulin G subclass 4; 274.RA.F4; RF mAb 61; mAb 61
UniProt
P0DOX5
Immunogen
The original antibody was produced by an Epstein Barr virus (EBV)-transformed B-cell line prepared from peripheral CD5+ blood lymphocytes of a Gm(a-), (x-), (b+), (g-), (f+) patient with classical rheumatoid arthritis (RA).
Application Notes
The binding activity of this antibody's original format (human IgM) to IgG Fc fragments was measured by ELISA, and a dissociation constant (Kd) of 6.0×10-7 M was calculated (Burastero et al., 1988; PMID: 3264319). This antibody exhibited strong reactivity with both monoclonal and polyclonal human anti-Rh IgG antibodies bearing the Gm(a) or Gm(g) specificity in hemagglutination assays (HAs), displaying a higher affinity for Gm(g). Additionally, it demonstrated efficient binding to heat-altered autologous Gm(a-), (g-) IgG and exhibited heteroclitic specificity at the clonal level in an autologous system (Williams Jr et al., 1992; PMID: 1380541). The binding of this antibody's original format to human IgG subclasses as measured by ELISA demonstrated that its binding specificity is IgG1~IgG2~IgG3>IgG4 (Duquerroy et al., 2007; PMID: 17395205).
Antibody First Published
Burastero et al. Monoreactive high affinity and polyreactive low affinity rheumatoid factors are produced by CD5+ B cells from patients with rheumatoid arthritis J Exp Med. 1988 Dec 1;168(6):1979-92. doi: 10.1084/jem.168.6.1979 PMID:3264319
Note on publication
The original publication explores the production of monoreactive high affinity and polyreactive low affinity rheumatoid factors by CD5+ B cells from patients with rheumatoid arthritis, and characterizes the binding activity and disassociation constant of several of the produced mAbs.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
