Cat. No.
MABL-1973
Application
SPR, ELISA
Isotype
Engineer antibody
Species Reactivity
Bovine, Cat, chimpanzee, Dog, Donkey, Goat, Guinea Pig, Horse, Rabbit, Rat, rhesus macaque, Sheep, Swine, Syrian Hamster, Human, Mouse
Clone No.
IgG-Fc-16
From
Recombinant Antibody
Specificity
This antibody is specific for the mammalian IgG-Fc domain. It was tested for and found to bind the human IgG-Fc domain (all subclasses). In a cross-reactivity test, it also bound bovine, mouse, rat, rabbit, dog, cat, swine, sheep, Chimpanzee, Rhesus macaque, donkey, horse, goat, Syrian hamster, and guinea pig IgG-Fcs.
Alternative Names
UniProt
Immunogen
The original antibody was generated by immunizing llamas with mammalian IgG antibodies and Fc fragments, creating the appropriate phage display library, and panning against the original target.
Application Notes
The reactivity of the original version of this antibody (camelid (llama) VHH) was tested using ELISA. Its binding affinity (KD) was measured using SPR and was found to be 13.1, 6.81, 16.2, 21.3, 1.70, 2.79, and 8.41 nM for human IgG, human IgG1, human IgG2, human IgG3, human IgG4, bovine IgG, and mouse IgG, respectively. Its binding to human, mouse, and bovine IgG was further verified via chromatography testing; the dynamic binding capacity of the antibody when immobilized onto NHS sepharose was 11.5 mg human IgG/ml matrix (US9040666B2).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
