Cat. No.
MABL-2002
Application
ELISA, FC, IF
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
AF6-78.25.4
From
Recombinant Antibody
Specificity
This antibody is specific for IgM (Igh-Cb and related haplotypes).
Alternative Names
IgM
UniProt
Immunogen
This antibody was raised by immunizing C57BL/6 mice with spleen cells.
Application Notes
PI- cells were used in an flow cytometry experiment using this antibody (Stall and Loken, 1984; pmid:6690619). B lymphoma mouse cells were used for flow cytometry using this antibody (Alés-martinez et al, 1988; pmid:3261870). The serum concentrations of a- and b-allotype IgM, IgG2a, and IgA were determined with ELISA using this antibody (Baumgarth et al, 1999; pmid:10051627). Single mouse cell suspensions from spleens and PerC were used for flow cytometery with this antibody (Baumgarth et al, 1999; pmid:10051627). pre-B cells, B lymphocytes, plasma cells, and hybridomas from mice were immunostained with this antibody (Velardi et al, 1984; pmid:6432906). Serum IgM from various mouse strains was used for ELISA with this antibody (Sieckmann et al, 1991; pmid:1709612). Single-cell suspensions of PeC were used in a flow cytometry experiment with this antibody (Thurnheer et al, 2003; pmid:12707334).
Antibody First Published
Stall et al. ALLOTYPIC SPECIFICITIES OF MURINE IgD AND IgM RECOGNIZED BY MONOCLONAL ANTIBODIES J Immunol. 1984 Feb;132(2):787-95. PMID:6690619
Note on publication
Hybridomas generated from mice immunized with allotype and H-2-incompatible spleen cells were screened by flow cytometry
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
