Cat. No.
MABL-2003
Application
affinity chromatography, IP, RIP, ELISA, FC, IF
Isotype
Engineer antibody
Species Reactivity
Trout
Clone No.
1.1399999999999999
From
Recombinant Antibody
Specificity
This antibody is specific for trout IgM. It reacts with the IgM heavy chain (Hedfors et al, 2012, pmid:23009920). It is not directed against nonprotein antigenic parts of the IgM, which is shown by the failure of IgM glycopeptides to inhibit precipitation (Deluca et al, 1983, pmid:6347695). 1.14 is crossreactive with atlantic salmon IgM (Hedfors et al, 2012, pmid:23009920). The antibody targets either IgM-A of IgM-B (Hedfors et al, 2012, pmid:23009920). It has been shown that this antibody does not show crossreactivity with catfish IgM (Moulana et al, 2008, pmid:18676026).
Alternative Names
Trout immunoglobulin M; immunoglobulin M
UniProt
Immunogen
This antibody was raised by immunizing female BALB/c mice with purified trout IgM.
Application Notes
This antibody has been used for affinity chromatography with the protein A (Deluca et al, 1983, pmid:6347695). The antibody was shown to be effective in flow cytometry application (Deluca et al, 1983, pmid:6347695). It was demonstrated that this antibody can be utilized in the immunofluorescence staining of trout head kidney, spleen and thymus cells (Deluca et al, 1983, pmid:6347695). Furthermore, it has been shown that this antibody can be used in the immunoprecepitation of trout IgM (Deluca et al, 1983, pmid:6347695). It is used in western blots as a negative against catfish IgL σ (Edholm et al, 2010, pmid:20149930) and MHC class IIβ (Moulana et al, 2008, pmid:18676026). Finally, 1.14 antibody can be used to detect trout antibody in an ELISA (Costa et al, 2011, pmid: 21641928).
Antibody First Published
Dominick DeLuca, Melanie Wilson and Gregory W. Warr Lymphocyte heterogeneity in the trout, Salmo gairdneri, defined with monoclonal antibodies to IgM* Eur. J. Immunol 13: 546-551. PMID:6347695
Note on publication
It describes the generation of the antibody and its use in research on the trout immune system.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
