Cat. No.
MABL-2015
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
3F5
From
Recombinant Antibody
Specificity
This antibody binds mouse IL-10. Interleukin 10 is a major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation.
Alternative Names
IL10; Interleukin-10; Interleukin 10; Cytokine synthesis inhibitory factor (CSIF)
UniProt
P18893
Immunogen
The original antibody was generated by immunizing New Zealand white rabbits with recombinant mouse IL-10 protein.
Application Notes
The original rabbit IgG antibody binds mouse IL-10 with a high affinity and has a dissociation constant (Kd) of 1.224×10^(-10) M. A combination of 2H4 and 3F5 can be used in a double antibody sandwich ELISA, where 2H4 is used as a capture antibody and 3F5 is used as a detection antibody. The sensitivity of mouse IL-10 detection for this pair was reported to be 1.965pg/mL (CN116396384).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
