Cat. No.
MABL-2021
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
12E 3
From
Recombinant Antibody
Specificity
This antibody binds human interleukin 12. It is required for early control of infection and for generation maintenance of acquired protective immunity.
Alternative Names
IL12; IL-12p70; IL12p70
UniProt
P29459; P29460
Immunogen
The original antibody was generated by immunizing New Zealand rabbits with recombinant human IL-12 protein containing Ile23-Ser328 sequence of IL-12A and Arg23-Ser219 sequence of IL-12B.
Application Notes
The original rabbit IgG antibody binds human recombinant interleukin-12 protein containing Ile23-Ser328 sequence of IL-12A and Arg23-Ser219 sequence of IL-12B with a binding affinity of Kd=1.95 nM. A combination of 12E3 and 7G11 can be used in a double antibody sandwich ELISA, where 12E3 is used as a capture antibody and biotin-labeled 7G11 is used as a detection antibody. The sensitivity of human IL-12 detection for this pair was reported to be 11.05pg/mL (11.05ng/L) (CN115819580).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
