Cat. No.
MABL-2022
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
7G11
From
Recombinant Antibody
Specificity
This antibody binds human interleukin 12. It is required for early control of infection and for generation maintenance of acquired protective immunity.
Alternative Names
IL12; IL-12p70; IL12p70
UniProt
P29459; P29460
Immunogen
The original antibody was generated by immunizing New Zealand rabbits with recombinant human IL-12 protein containing Ile23-Ser328 sequence of IL-12A and Arg23-Ser219 sequence of IL-12B.
Application Notes
The original rabbit IgG antibody binds human recombinant interleukin-12 protein containing Ile23-Ser328 sequence of IL-12A and Arg23-Ser219 sequence of IL-12B with a binding affinity of Kd=2.14 nM. A combination of 12E3 and 7G11 can be used in a double antibody sandwich ELISA, where 12E3 is used as a capture antibody and biotin-labeled 7G11 is used as a detection antibody. The sensitivity of human IL-12 detection for this pair was reported to be 11.05pg/mL (11.05ng/L) (CN115819580).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
