Cat. No.
MABL-2027
Application
inhibition, neutralization, SPR, ELISA
Isotype
Engineer antibody
Species Reactivity
Human, Cynomolgus Monkey
Clone No.
6A1
From
Recombinant Antibody
Specificity
This antibody is specific for human IL-13. It cross-reacts with cynomolgus monkey IL-13.
Alternative Names
IL13; Interleukin-13; P600
UniProt
P35225
Immunogen
The original antibody was generate by immunizing SJL mice with E. Coli-produced recombinant human IL-13.
Application Notes
The original format of this antibody (mouse IgG1) showed binding activity against E.coli-expressed recombinant human IL-13 in a sandwich ELISA with an EC50 of 0.049 and 0.039 µg/ml for human and cynomolgus IL-13. The antibody also exhibited potent binding and neutralization activities against various forms of IL-13. In inhibition assays, the antibody showed IC50 values of 0.165 µg/ml for blocking human IL-13 binding to IL-13Rα1 and 0.056 µg/ml for IL-13 binding to IL-13Rα2. Moreover, it demonstrated strong neutralization capabilities with ND50 (neutralizing dose) values of 0.0783 µg/ml for human IL-13 and 0.04 µg/ml for cynomolgus IL-13, indicating its efficacy in inhibiting IL-13-induced TF-1 cell proliferation (where the ND50 value is the concentration of monoclonal antibody required to reduce TF-1 cell proliferation by 50%, in response to a set concentration of IL-13). Furthermore, the antibody effectively neutralized mammalian-expressed human IL-13 with an ND 50 value of 0.037 µg/ml and a recombinant Q130 human IL-13 variant with an ND50 value of 0.110 µg/ml. The binding affinity of the antibody was measured by SPR and found to be 32, 270, and 61.2 pM for det-1 tagged human IL-13, human IL-13, and cynomolgus IL-13, respectively (EP1802658B1).
Antibody First Published
Note on publication
The original publication describes the generation and characterization of an anti- human IL-13 antibody and its derivatives, including its humanization.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
