Cat. No.
MABL-2028
Application
neutralize, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
CNTO607
From
Recombinant Antibody
Specificity
This antibody binds an epitope at the surface formed by helices A and D of human interleukin- 13. This epitope overlaps with the IL-4Ralpha/IL-13Ralpha1 receptor-binding site, which explains the neutralizing effect of CNTO607. IL-13 is a cytokine secreted by T helper type 2 (Th2) cells, CD4 cells, natural killer T cell, mast cells, basophils, eosinophils and nuocytes. It inhibits the production of inflammatory cytokines (IL-1, IL-6, TNF, IL-8) by LPS-stimulated monocytes. It synergizes with IL2 in regulating interferon- gamma synthesis. It is a mediator of allergic inflammation and different diseases including asthma.
Alternative Names
IL13; NC30; Interleukin-13; MOR3406
UniProt
P35225
Immunogen
The original antibody was generated from Morphosys HuCAL-Gold™ phage display library after panning against an IL-13 variant (R130Q) identified in a sub-population of patients who suffer from asthmatic attacks.
Application Notes
The original IgG1 version of this antibody binds human IL-13 with an affinity of 18pM and prevents binding of IL-13 to both IL-13Rα1 and IL-4Rα. This antibody can bind native wild type and recombinant human IL-13 in an ELISA (PMID: 19361524). This antibody is capable of neutralizing IL13 activity in the TF-1 and in the IL-13Rα1 receptor binding assay (US20080044420). This antibody has the tendency to self associate. The IgG1 version of this antibody can be concentrated to ∼13 mg/ml before irreversible precipitation occurs (PMID: 22915597). The relative binding affinity of the IgG1 version of this antibody was determined to be 18.4 pM (PMID: 22915597).
Antibody First Published
Teplyakov et al. Epitope mapping of anti-interleukin-13 neutralizing antibody CNTO607. J Mol Biol. 2009 May 29;389(1):115-23. PMID:19361524
Note on publication
Describes the epitope mapping of this antibody using X-ray crystallography.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
