Cat. No.
MABL-2030
Application
neutralize, therapeutic, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
M1295
From
Recombinant Antibody
Specificity
This antibody binds human interleukin-13 at the surface of helices A and D close to N- and C- termini of the IL-13 polypeptide chain. IL-13 is a cytokine secreted by T helper type 2 (Th2) cells, CD4 cells, natural killer T cell, mast cells, basophils, eosinophils and nuocytes. It inhibits the production of inflammatory cytokines (IL-1, IL-6, TNF, IL-8) by LPS-stimulated monocytes. It synergizes with IL2 in regulating interferon-gamma synthesis. It is a mediator of allergic inflammation and different diseases including asthma.
Alternative Names
IL13; NC30; Interleukin-13; hC836; m836; H2L6; LC6HC2; HFAL62
UniProt
P35225
Immunogen
The original mouse antibody m836 was generated by immunizing BALB/c mice with a natural variant of human IL-13 known as R130Q. The humanized version H2L6 was generated by grafting CDRs of mouse antibody onto selected human heavy chain and light chain frameworks and finally site-directed mutagenesis of two methionine in the CDRs of heavy chain with binding affinity optimization resulted in the generated of M1295.
Application Notes
This antibody can bind human IL13 in an ELISA. The affinity of original IgG1 antibody for wild type human IL-13 was determined by BIAcore to be 5.7 ± 1.3 pM (PMID: 20226193). Preclinical studies confirm the functional effects of M1295-mediated neutralization of human IL-13 in human cell based in vitro assays using mechanistically relevant endpoints associated with the pathogenesis of asthma, atopic dermatitis, and eosinophil-mediated disease (US8399630).
Antibody First Published
Fransson et al. Human framework adaptation of a mouse anti-human IL-13 antibody. J Mol Biol. 2010 Apr 30;398(2):214-31. PMID:20226193
Note on publication
Describes the humanization and site directed affinity maturation of an anti-IL13 antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
