Anti-IL-17 mAbs [Antibody 7](MABL-2033)

MABL-2033

crystallization, functional assays, in vivo, SPR, ELISA

Engineer antibody

Dog, Human, Cynomolgus Monkey

Antibody 7

Recombinant Antibody

This antibody is specific for IL-17; it binds residues 74 (Leu), 85 (Tyr), 86 (H is), and 87 (Met) of the mature IL-17 sequence. In humans, It has been shown to bind both IL-17A and IL-17F.

IL17; IL-17A; IL17A; CTLA8; Interleukin-17A; IL-17A; Cytotoxic T- lymphocyte-associated antigen 8; CTLA-8; IL17F; IL-17F; Interleukin-17F; IL-17F; Cytokine ML-1

The original antibody was produced by immunizing Balb/c mice with purified recombinant human IL-17.

The IC50 values for this antibody as measured in an HT1080 assay in response to 1 nM human or cynomolgus monkey (cyno) IL-17A were 0.8 ± 0.2 nM and 4.8 ± 1.6 nM, respectively. Partial inhibition was also observed with IL-17F. Further assays showed cross-reactivity with dog IL-17A. This antibody neutralized the synergistic IL-6 response as measured in an altered human HT1080 assay with an IC50 value of 0.24 ± 0.05 nM. pA2 = 9.23 and Kd = 663 pM were measured when testing the affinity of this antibody for human IL-17A. Further kinetic analysis on recombinant human IL-17A (E. coli), cyno IL-17A (E. coli), and human IL-17A-Flag-His10 (HEK) yielded a 1:1 Langmuir KD and Bivalent Analyte KD of 0.195 nM, 0.512 nM, and 0.171 nM, and 2.098 nM, 1.340 nM, and 0.41-1.88 nM, appropriately. The biological activity of this antibody was tested extensively in the air pouch of Balb/c; it effectively inhibited IL-17A-induced IL-6 release and the influx of white blood cells in the air pouch, as reflected by low ID50 values. This antibody exhibited inhibitory effects on the IL-17A/TNFα synergized responses in post-mortem cartilage explants and synovial fibroblasts with the potential to modulate inflammatory and destructive responses induced by the synergistic action of IL-17A and TNFα. X-ray crystallography of the IL-17A bound Fab version of this antibody indicated that it binds residues 74 (Leu), 85 (Tyr), 86 (H is), and 87 (Met) of the mature sequence (US8865166).

100 μg Purified antibody.

1 mg/ml.

Protein A affinity purified

PBS with 0.02% Proclin 300.

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.