Cat. No.
MABL-2032
Application
WB, ELISA, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
14.1
From
Recombinant Antibody
Specificity
The antibody is specific for IL-16.
Alternative Names
LCF; interleukin 16; NPRprIL-16; IL16
UniProt
Q14005
Immunogen
The antibody was raised against IL-16 using a synthetic peptide corresponding to the predicted amino acid sequence of a hydrophilic region of IL-16, conjugated to keyhole limpet hemocyanin.
Application Notes
The antibody has been used in Western blot analysis to detect IL-16. It has been used in ELISA-type binding assays. The antibody has been used to stain endogenous IL-16 in sheets from murine ear skin after intradermal injection of TNF-α, and in flow cytometry to detect IL-16 on epidermal cells and emigrated cutaneous DC (Stoitzner et al, 2001).
Antibody First Published
Cruikshank et al. Molecular and functional analysis of a lymphocyte chemoattractant factor: association of biologic function with CD4 expression. Proc Natl Acad Sci U S A. 1994 May 24;91(11):5109-13. PMID:7910967
Note on publication
Describes the original generation of this antibody and its use in molecular cloning of IL-16.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
