Cat. No.
MABL-2036
Application
WB, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Chicken
Clone No.
1G8
From
Recombinant Antibody
Specificity
The antibody binds specifically to chicken IL-17, a proinflammatory cytokine produced by activated T cells. Further, The antibody binds to duck IL-17.
Alternative Names
Interleukin 17A; chIL-17; Interleukin-17A; IL-17; chicken IL-17A
UniProt
B4ER12
Immunogen
The original antibody was raised by immunizing BALB/C mice with recombinant chicken IL-17.
Application Notes
The specificity of this antibody (IgG1) was confirmed in ELISA analysis. The antibody detected the recombinant chIL-17A by western blot. This antibody was used for flow cytometry on CU205 cells (Yoo et al, 2008; pmid: 18022248). The antibody was used to study the interactions between chIL-17RA and chIL-17A by western blot (Kim et al, 2014; pmid: 24980970). The cross-reactivity of the antibody to chIL17 with duck IL17 was evaluated by indirect ELISA. The binding intensity of the antibody to recombinant chIL17 was similar to recombinant duIL17. The antibody detected duIL-17A by western blot (Yoo et al., 2009; pmid: 19573930).
Antibody First Published
Yoo et al. Monoclonal antibodies reactive with chicken interleukin‐17. Vet Immunol Immunopathol. 2008;121(3‐4):359 ‐363. PMID:18022248
Note on publication
The article describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
