Cat. No.
MABL-2049
Application
functional assay, ELISA
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
9H10
From
Recombinant Antibody
Specificity
This antibody binds mouse Interleukin-1 alpha, which is a cytokine constitutively present intracellularly in nearly all resting non-hematopoietic cells that plays an important role in inflammation and bridges the innate and adaptive immune systems. IL-1α is produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells.
Alternative Names
IL1A; IL-1α; Interleukin-1 alpha; IL-1 alpha
UniProt
P01582
Immunogen
The original antibody was generated by immunizing IL-1α/β double KO mice with mouse IL-1 alpha.
Application Notes
The original mouse IgG antibody binds mouse IL-1A with a binding affinity of Kd= 0.17 nM. The binding characterization of this antibody was done using ELISA. In vitro antibody potency was determined by its ability to inhibit IL-8 production by MRC-5 cells. This antibody was able to inhibit IL-8 production by MRC-5 cells with an IC50 of 2.00E−10M. This antibody was used in combination with 10G11 (anti- mouse IL-1B) antibody for the generation of anti-mouse IL-1α/β DVD-Ig molecule (US7612181).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
