Cat. No.
MABL-2051
Application
IH, neutralizing, WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Chicken
Clone No.
1
From
Recombinant Antibody
Specificity
The antibody is specific for chicken IL-1β, a pleiotropic molecule with a wide spectrum of activities including metabolic, physiologic, hematologic, and immunologic effects. The antibody did not react with chicken IL-18.
Alternative Names
Interleukin IL1‐beta; chIL-1B-1; Interleukin-1; chIL-1B
UniProt
O73909
Immunogen
The original antibody was raised immunising BALB/C mice with recombinant chicken IL-1B protein.
Application Notes
The specificity of the original format of this antibody was confirmed by ELISA analysis (Lee et al, 2014; pmid: 25037821). The antibody detected chIL-1β protein by Western blot analysis. The antibody was used as a capture antibody for measuring serum IL-1B levels in chicken by capture ELISA analysis. The original format of the antibody was used to localize IL-1β-secreting cells in chicken immune tissues. The antibody neutralized the bioactivity of chIL-1B-induced cell proliferation and NO production by primary thymocytes (Lee et al, 2014; pmid: 25037821).
Antibody First Published
Lee et al. Development and characterization of mouse monoclonal antibodies reactive with chicken IL-1β Poult Sci. 2014;93(9):2193‐2198. PMID:25037821
Note on publication
The paper describes the generation and characterization of the antibody, which is specific for chIL-1β.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
