Cat. No.
MABL-2055
Application
inhibit, FC
Isotype
Engineer antibody
Species Reactivity
Human, Cynomolgus Monkey
Clone No.
Antibody 6
From
Recombinant Antibody
Specificity
This antibody binds human IL-1R1 and cynomolgus monkey IL-1R1 but does not cross react with mouse IL-1R1. This antibody binds an epitope containing amino acids in a discontinuous epitope of 33 amino acids in three regions of human IL-1R1; N123-V134, L140-K157 and K178-R180. The human IL-1R1 protein acts as a receptor for IL1A, IL1B and IL1RN.
Alternative Names
CD121a; IL1R1; IL1R; IL1RA; IL1RT1;Interleukin-1 receptor type 1; IL-1R- 1; IL-1RT-1; IL-1RT1; CD121 antigen-like family member A; Interleukin-1 receptor alpha; IL-1R-alpha; Interleukin-1 receptor type I; p80
UniProt
P14778
Immunogen
The original antibody was isolated from a naïve human single chain Fv (scFv) phage display libraries using a series of selection cycles on recombinant human IL-1R1. The scFv was later reformatted to the human IgG1 version.
Application Notes
The binding affinity of this antibody to human IL-1R1 was determined using kinetic exclusion assay. It was reported that the human IgG1 version of this antibody binds human IL-1R1 with a binding affinity of 3.05 pM. The human IgG1 version of this antibody shows the inhibition of IL-1β induced IL- 8 release from HeLa cells. The IgG2 version was capable of inhibition of IL-1β and IL-α induced IL-8 release from HeLa cells. The binding of this antibody was capable of inhibiting binding of IL-1Ra binding to IL-1R1. This antibody was reported to inhibit huIL-1β-induced IL-8 release from CYNOM-K1 cells. The human IgG1 and IgG2 versions of this antibody was capable of inhibiting IL-6 production in human whole blood. The IgG2 version also blocked IL-1 activity (US8298533).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
