Cat. No.
MABL-2058
Application
IP, NTRL, ELISA
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
Jes6-1
From
Recombinant Antibody
Specificity
This antibody binds murine IL-2.
Alternative Names
Interleukin-2; IL2; T cell growth factor; JES6-1A12
UniProt
P04351
Immunogen
Rat antibodies were raised against murine IL-2.
Application Notes
JES6-1 is a scFv reformat of the variable domain sequences of the JES6-1 monoclonal antibody (Spangler et al, 2015). It was shown in this study that JES6-1 scFv binding to mouse IL-2 would sterically hinder interaction with the IL-2 receptor, thereby blocking downstream signalling functions. This antibody is well known as a neutralising antibody against mouse IL-2, and is useful for cytokine assays such as ELISA (Abrams et al, 1992).
Antibody First Published
Abrams et al. Strategies of Anti-Cytokine Monoclonal Antibody Development: Immunoassay of IL-10 and IL-5 in Clinical Samples Immunological Reviews 1992, No. 127 PMID:1387110
Note on publication
Used the anti-mouse IL-2 clone JES6-1A12 to assay the presence of mouse IL-2 in enzyme-linked assays.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
