Cat. No.
MABL-2059
Application
crystallization, FACS, Latex agglutination, WB, ELISA, FC, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
LNKB-2
From
Recombinant Antibody
Specificity
This antibody binds human IL-2 at the 59-72 site of the IL-2 sequence.
Alternative Names
Interleukin 2; LNKB2
UniProt
P60568
Immunogen
Application Notes
This antibody was quantified by ELISA and used for latex-agglutination analysis of human IL-2. It was measured to bind human IL-2 with high affinity (Ka ≈ 3 × 108 M-1) (Lunev et al., 1990; PMID: 1718463). This antibody has been used successfully for WB and FACS (Stratieva-Taneeva et al., 1993; PMID: 8359822). The crystal structure characterization of the Fab version of this antibody was performed (Fokin et al., 2000; PMID: 11040993). Further crystallization of the Fab version of this antibody with an antigenic peptide revealed that the antibody-antigen complexation involves a significant rearrangement of the epitope-containing region of the IL-2 with retention of the α-helical character of the epitope fragment (Afonin et al., 2001; PMID: 11468348).
Antibody First Published
Lunev et al. Latex-agglutination analysis of human recombinant interleukin- 2 with monoclonal antibodies Biomed Sci. 1990 Jan;1(1):68-72 PMID:1718463
Note on publication
The original publication describes the generation and characterization of monoclonal antibodies to human interleukin-2.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
